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文章标题：姜黄素通过抑制TLR3/TBK1/IFNB级联反应减弱聚肌胞诱导的小鼠巨噬细胞免疫和炎症反应

摘要：姜黄素因其对炎症和免疫途径的有益作用而被广泛用于膳食补充剂。为了更好地了解姜黄素在病毒感染过程中的作用，我们通过转录组深度测序、实时荧光定量 PCR、western blotting 和 ELISA 等实验验证，评估其对聚肌胞诱导的小鼠巨噬细胞(RAW264.7)的影响。

我们的研究结果有助于更好地理解姜黄素在病毒感染过程中的作用，表明姜黄素制剂具有支持健康免疫系统的潜力。

该论文发表于《Journal of Functional Foods 》2022年2月89卷第104949条

姜黄素因其对炎症和免疫途径的有益作用而被广泛用于膳食补充剂。为了更好地了解姜黄素在病毒感染过程中的作用，我们通过转录组深度测序、实时荧光定量PCR、westernblotting和ELISA等实验验证，评估其对聚肌胞诱导的小鼠巨噬细胞（RAW264.7）的影响。转录组学结果显示，157个差异表达基因与姜黄素对聚肌胞诱导的巨噬细胞的反应活性有关。通过生物信息学分析，揭示了Toll样受体，特别是Toll样受体3（TLR3）参与了姜黄素对聚肌胞诱导的免疫和炎症反应的影响。实时荧光定量PCR结果显示，姜黄素(2.5、5、10μM)显著下调Tlr3、Irf-7、Ifnb1、Il-6、Tnfα和Il1bf的mRNA表达，且呈剂量依赖性。在westernblot或ELISA实验结果中也观察到姜黄素（5μM）显著下调TLR3（p<0.05）、TBK1（p<0.005）、IRF3（p<0.0005）、IRF7（p<0.0005）、IFN-β（p<0.0005）、TNF-α（p<0.0001）和IL6（p<0.0001）的蛋白或磷酸化蛋白的表达。综上所述，下调TLR3/TBK1/IFN-β级联反应可能是姜黄素减轻病毒样感染的潜在机制。

我们的研究结果有助于更好地理解姜黄素在病毒感染过程中的作用，表明姜黄素制剂具有支持健康免疫系统的潜力。六年潜心研究，只为给你呈上最优质的营养。

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Journal of Functional Foods 89 (2022) 104949

Curcumin attenuates poly(I:C)-induced immune and inflammatory responses in mouse macrophages by inhibiting TLR3/TBK1/IFNB cascade

Xue Zhang, Liang Chen, Chun Hu, David Fast, Lu Zhang, Binrui Yang, Juntao Kan, Jun Du

Amway Innovation & Science, 720 Cailun Road, Shanghai 201203, China

Nutrilite Health Institute, Amway Innovation & Science, 5600 Beach Boulevard, Buena Park, CA 90621, USA

Amway Innovation & Science, 7575 Fulton Street, Ada, MI 49355, USA

Keywords: Curcumin Poly(I:C) RAW264.7 macrophages TLR3 Viral infection

ABSTRACT

Curcumin is widely used in dietary supplements due to its beneficial effects on inflammatory and immune pathways. To better understand the action of curcumin during viral infection, its effect on stimulation of RAW264.7 macrophages with polyinosinic-polycytidylic acid (poly(I:C)) was evaluated by deep sequencing transcriptome, followed by RT-qPCR, western blotting and ELISA validations. The transcriptomics results showed that 157 differentially expressed genes are in association with the activity of curcumin on the cellular response to poly(I:C) stimulation. Gene Ontology and KEGG enrichment analysis uncovered the involvement of TLRs, in particular TLR3, in curcumin's effect on poly(I:C)-induced immune and inflammatory responses. RT-qPCR results showed that curcumin (2.5, 5, 10 μM) significantly down-regulated the mRNA expression of Tlr3, Ifnb1, Il6, Tnfα, and Il1β in a dose-dependent manner. Significant down-regulation of curcumin (5 M) on protein or phosphorylated protein expressions of TLR3 (p < 0.05), TBK1 (p < 0.005), IRF3 (p < 0.0005), IRF7 (p < 0.005), IFN-β (p < 0.0005), TNF-α (p < 0.0001) and IL6 (p < 0.0001) were also observed in western blotting or ELISA results. Taken together, these results indicated that down-regulation of TLR3/TBK1/IFN-β cascade may involve in the underlying mechanism of curcumin alleviating virus-like infections. The findings from our study contribute to a better understanding of the action of curcumin during viral infection, suggesting the potential of curcumin preparations to support a healthy immune system.

姜黄素因其对炎症和免疫途径的有益作用而被广泛用于膳食补充剂。为了更好地了解姜黄素在病毒感染过程中的作用，我们通过转录组深度测序实时荧光定量 PCR、western blotting 和 ELISA 等实验验证，评估其对聚肌胞诱导的小鼠巨噬细胞(RAW264.7)的影响。

我们的研究结果有助于更好地理解姜黄素在病毒感染过程中的作用，表明姜黄素制剂具有支持健康免疫系统的潜力。

姜黄素通过抑制TLR3/TBK1/IFNB级联反应减弱聚肌胞诱导的小鼠巨噬细胞免疫和炎症反应

该论文发表于《Journal of Functional Foods》2022年2月89卷第104949条整篇论文请去安利头条 App-下载专区下载

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Journal of Functional Foods

journal homepage: www.elsevier.com/ocate/jff